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Axon Medchem LLC small-molecule inhibitors chir99021 ch
Small Molecule Inhibitors Chir99021 Ch, supplied by Axon Medchem LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
small-molecule inhibitors chir99021 ch - by Bioz Stars, 2026-11
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Recombinant:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).

Cell Culture:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).

Transfection:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).

RNA Sequencing:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).

Construct:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).

Plasmid Preparation:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).

Software:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).

In Vitro:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).

Suspension:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).

Microscopy:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).

Protease Inhibitor:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).

Methylation:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).

Purification:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).

DNA Methylation Assay:

Article Title: Methods of generating a synthetic embryo
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—Axon Medchem 1408).

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “2i” inhibitors the following agents were added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: A programmable system to methylate and demethylate N 6 -methyladenosine (m 6 A) on specific RNA transcripts in mammalian cells
Article Snippet: Naïve conditions for mESC included 10 μg recombinant human LIF (Peprotech) and small-molecule inhibitors CHIR99021 (CH, 1–3 μM; Axon Medchem) and PD0325901 (PD, 1 μM; TOCRIS) termed 2i. mESCs were cultured on fibronectin-coated plates where indicated.

Article Title: Isolated naive pluripotent stem cells and methods of generating same
Article Snippet: Where indicated “21” the following was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 1 μM—TOCRIS).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3μM- Axon Medchem 1386) and PD0325901 (PD, 1μM — Axon Medchem 1408) (referred to as 2i).

Article Title: Methods of generating a synthetic embryo
Article Snippet: 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM-Axon Medchem) and PD0325901 (PD, 11 μM—Axon Medchem 1408). (ii) alternative 2i/LIF conditions (a2i or a2i-Lif conditions): serum-free chemically defined N2B27-based media: 240 ml Neurobasal (ThermoFisher 21103049) and 240 ml of DMEM-F12 with HEPES (SIGMA D6421), 5 ml N2 supplement (Invitrogen; 17502048), 5-10 ml B27 supplement (Invitrogen; 17504044), 2 mM glutamine or 1 mM GLutamax (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), 1% penicillin-streptomycin (Invitrogen).

Article Title: Principles of signaling pathway modulation for enhancing human naive pluripotency induction
Article Snippet: Where indicated 2i was added: small-molecule inhibitors CHIR99021 (CH, 3 μM- Axon Medchem) and PD0325901 (PD, 1 μM – Axon Medchem 1408).

Article Title: Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.
Article Snippet: Naive 2i/Lif conditions for murine PSCs included 20 ng/mL recombinant human Lif (in-house made), small-molecule inhibitors CHIR99021 (CHIR, 3mM- Axon Medchem 1386) and PD0325901 (PD, 1mM — Axon Medchem 1408) (referred to as 2i).



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